Descripción
The initial goal of the project is to detect new alien species in early establishment phase in Norwegian nature. Insects and other arthropods have been sampled from urban and suburban sites in southeast-Norway. The trap collects flying insects passively in a bottle with 96% ethanol and is emptied once every four weeks, from May to September. All arthropods in the samples are identified by DNA-metabarcoding. DNA is extracted from the samples using a soft lysis method, which extracts DNA from the specimens without destroying them. The COI-region is then amplified from the extracted DNA and sequenced. The sequences are then filtered and quality checked and identified through matching with an in-house database of reference sequences.
Registros
Los datos en este recurso de evento de muestreo han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 974 registros.
también existen 1 tablas de datos de extensiones. Un registro en una extensión provee información adicional sobre un registro en el core. El número de registros en cada tabla de datos de la extensión se ilustra a continuación.
Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Jacobsen R, Åström J (2024). Early detection of alien arthropod species in Norway. Version 1.0. Norwegian Institute for Nature Research. Samplingevent dataset. https://ipt.nina.no/resource?r=early_detection_alien_arthropods&v=1.0
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es Norwegian Institute for Nature Research. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: 1baa2c29-e247-4158-8cd4-05b72db1dd4e. Norwegian Institute for Nature Research publica este recurso y está registrado en GBIF como un publicador de datos avalado por GBIF Norway.
Palabras clave
Samplingevent
Contactos
- Proveedor De Los Metadatos ●
- Originador ●
- Punto De Contacto
https://orcid.org/0000-0003-4055-1096
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- Originador ●
- Punto De Contacto
Cobertura geográfica
As of 2023, the geographic scope is limited to the wider Oslo bay area.
| Coordenadas límite | Latitud Mínima Longitud Mínima [58,993, 9,637], Latitud Máxima Longitud Máxima [60,273, 11,374] |
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Cobertura taxonómica
The sampling and bioinformatics focus on insects, but other classes of Arthropods also occur in the dataset.
| Reino | Metazoa |
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| Filo | Arthropoda |
Cobertura temporal
| Fecha Inicial / Fecha Final | 2021-05-05 / 2023-08-24 |
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Datos del proyecto
The main goal of the project is to detec new alien species in early establishment phase in Norwegian nature.
| Título | Early detection of alien arthropod species in Norway |
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| Fuentes de Financiación | The project is funded by the Environmental Agency of Norway. |
| Descripción del área de estudio | The survey is centred around the Oslofjord in southeast Norway. |
| Descripción del diseño | Sites are selected by two different methods, both aiming to target areas with high probability for occurrence of alien species, in particular new alien species. All sites based on the SSB grid for Norway, and formed as 250 x 250 meter squares. The automatically selected sites are drawn by an algorithm based on the following premises; (1) minimum eight single houses in the site (2) population density of minimum 30 and maximum 125 in the site (3) minimum 100 meters to the closest forested area (based on AR5 – the rationale for this was to ensure there was some nature in the site to which alien species could spread, as species in gardens or parks would not be included) (4) a weighted likelihood based on the modelled occurrence of alien plants from Olsen et al. (2017), i.e. higher likelihood to include sites in hot-spots for alien plants. The first two criteria resulted in many sites in residential areas with gardens, which was the intention, as imported garden plants are a very important source of alien species (Artsdatabanken 2023a). The manually selected sites are placed near potential pathways of introduction and spread of alien species that are not covered by the automatically selected sites. These sites are typically close to waste disposal sites with open depot for garden waste, transport hubs such as docks or freight terminals, or timber processing sites that are still or have previously imported timber from abroad (as timber can house several alien species of insects). |
Personas asociadas al proyecto:
Métodos de muestreo
One malaise trap per site
| Área de Estudio | Southeast Norway Sampling conducted in May to September |
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Descripción de la metodología paso a paso:
- Sampling arthropods with malaisetraps, in 96% ethanol, emptied once every four weeks Straining the ethanol from the samples and adding control organisms (10 meal worms, 3 crickets and 10 bruchinae beetles). Lysis of the samples with ATL buffer and proteinase-K (100mL ATL = 1mL proteinase-K), incubated 3,5 hours while shaking (120 RPM) at 56 degrees celsius. DNA exctracted with Blood and Tissue kit (Quiagen) from 200 µL of the buffer solution. Amplifying COI with the primers BF3-BR2 (Elbrecht et al. 2019) in PCR (22 cycles) with overhang adaptors sequences in first run and Illumina-indexes in second run. Quality control of PCR products on a Tape Station (Agilent 4200) and cleaning with MAG-BIND RXN PURE PLUS. Sequencing with a Illumina NovaSeq machine at Norwegian Sequencing Centre (NSC) in Oslo.
Metadatos adicionales
| Identificadores alternativos | https://ipt.nina.no/resource?r=early_detection_alien_arthropods |
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