Descrição
The initial goal of the project is to detect new alien species in early establishment phase in Norwegian nature. Insects and other arthropods have been sampled from urban and suburban sites in southeast-Norway. The trap collects flying insects passively in a bottle with 96% ethanol and is emptied once every four weeks, from May to September. All arthropods in the samples are identified by DNA-metabarcoding. DNA is extracted from the samples using a soft lysis method, which extracts DNA from the specimens without destroying them. The COI-region is then amplified from the extracted DNA and sequenced. The sequences are then filtered and quality checked and identified through matching with an in-house database of reference sequences.
Registros de Dados
Os dados deste recurso de evento de amostragem foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 974 registros.
Também existem 1 tabelas de dados de extensão. Um registro de extensão fornece informações adicionais sobre um registro do núcleo. O número de registros em cada tabela de dados de extensão é ilustrado abaixo.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versões
A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.
Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Jacobsen R, Åström J (2024). Early detection of alien arthropod species in Norway. Version 1.0. Norwegian Institute for Nature Research. Samplingevent dataset. https://ipt.nina.no/resource?r=early_detection_alien_arthropods&v=1.0
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é Norwegian Institute for Nature Research. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 1baa2c29-e247-4158-8cd4-05b72db1dd4e. Norwegian Institute for Nature Research publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Norway.
Palavras-chave
Samplingevent
Contatos
- Provedor Dos Metadados ●
- Originador ●
- Ponto De Contato
https://orcid.org/0000-0003-4055-1096
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- Originador ●
- Ponto De Contato
Cobertura Geográfica
As of 2023, the geographic scope is limited to the wider Oslo bay area.
| Coordenadas delimitadoras | Sul Oeste [58,993, 9,637], Norte Leste [60,273, 11,374] |
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Cobertura Taxonômica
The sampling and bioinformatics focus on insects, but other classes of Arthropods also occur in the dataset.
| Reino | Metazoa |
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| Filo | Arthropoda |
Cobertura Temporal
| Data Inicial / Data final | 2021-05-05 / 2023-08-24 |
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Dados Sobre o Projeto
The main goal of the project is to detec new alien species in early establishment phase in Norwegian nature.
| Título | Early detection of alien arthropod species in Norway |
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| Financiamento | The project is funded by the Environmental Agency of Norway. |
| Descrição da Área de Estudo | The survey is centred around the Oslofjord in southeast Norway. |
| Descrição do Design | Sites are selected by two different methods, both aiming to target areas with high probability for occurrence of alien species, in particular new alien species. All sites based on the SSB grid for Norway, and formed as 250 x 250 meter squares. The automatically selected sites are drawn by an algorithm based on the following premises; (1) minimum eight single houses in the site (2) population density of minimum 30 and maximum 125 in the site (3) minimum 100 meters to the closest forested area (based on AR5 – the rationale for this was to ensure there was some nature in the site to which alien species could spread, as species in gardens or parks would not be included) (4) a weighted likelihood based on the modelled occurrence of alien plants from Olsen et al. (2017), i.e. higher likelihood to include sites in hot-spots for alien plants. The first two criteria resulted in many sites in residential areas with gardens, which was the intention, as imported garden plants are a very important source of alien species (Artsdatabanken 2023a). The manually selected sites are placed near potential pathways of introduction and spread of alien species that are not covered by the automatically selected sites. These sites are typically close to waste disposal sites with open depot for garden waste, transport hubs such as docks or freight terminals, or timber processing sites that are still or have previously imported timber from abroad (as timber can house several alien species of insects). |
O pessoal envolvido no projeto:
Métodos de Amostragem
One malaise trap per site
| Área de Estudo | Southeast Norway Sampling conducted in May to September |
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Descrição dos passos do método:
- Sampling arthropods with malaisetraps, in 96% ethanol, emptied once every four weeks Straining the ethanol from the samples and adding control organisms (10 meal worms, 3 crickets and 10 bruchinae beetles). Lysis of the samples with ATL buffer and proteinase-K (100mL ATL = 1mL proteinase-K), incubated 3,5 hours while shaking (120 RPM) at 56 degrees celsius. DNA exctracted with Blood and Tissue kit (Quiagen) from 200 µL of the buffer solution. Amplifying COI with the primers BF3-BR2 (Elbrecht et al. 2019) in PCR (22 cycles) with overhang adaptors sequences in first run and Illumina-indexes in second run. Quality control of PCR products on a Tape Station (Agilent 4200) and cleaning with MAG-BIND RXN PURE PLUS. Sequencing with a Illumina NovaSeq machine at Norwegian Sequencing Centre (NSC) in Oslo.
Metadados Adicionais
| Identificadores alternativos | https://ipt.nina.no/resource?r=early_detection_alien_arthropods |
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