說明
The initial goal of the project is to detect new alien species in early establishment phase in Norwegian nature. Insects and other arthropods have been sampled from urban and suburban sites in southeast-Norway. The trap collects flying insects passively in a bottle with 96% ethanol and is emptied once every four weeks, from May to September. All arthropods in the samples are identified by DNA-metabarcoding. DNA is extracted from the samples using a soft lysis method, which extracts DNA from the specimens without destroying them. The COI-region is then amplified from the extracted DNA and sequenced. The sequences are then filtered and quality checked and identified through matching with an in-house database of reference sequences.
資料紀錄
此資源sampling event的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 974 筆紀錄。
亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Jacobsen R, Åström J (2024). Early detection of alien arthropod species in Norway. Version 1.0. Norwegian Institute for Nature Research. Samplingevent dataset. https://ipt.nina.no/resource?r=early_detection_alien_arthropods&v=1.0
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 Norwegian Institute for Nature Research。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 1baa2c29-e247-4158-8cd4-05b72db1dd4e。 Norwegian Institute for Nature Research 發佈此資源，並經由GBIF Norway同意向GBIF註冊成為資料發佈者。
關鍵字
Samplingevent
聯絡資訊
- 元數據提供者 ●
- 出處 ●
- 連絡人
https://orcid.org/0000-0003-4055-1096
- ●
- 出處 ●
- 連絡人
地理涵蓋範圍
As of 2023, the geographic scope is limited to the wider Oslo bay area.
| 界定座標範圍 | 緯度南界 經度西界 [58.993, 9.637], 緯度北界 經度東界 [60.273, 11.374] |
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分類群涵蓋範圍
The sampling and bioinformatics focus on insects, but other classes of Arthropods also occur in the dataset.
| Kingdom | Metazoa |
|---|---|
| Phylum | Arthropoda |
時間涵蓋範圍
| 起始日期 / 結束日期 | 2021-05-05 / 2023-08-24 |
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計畫資料
The main goal of the project is to detec new alien species in early establishment phase in Norwegian nature.
| 計畫名稱 | Early detection of alien arthropod species in Norway |
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| 經費來源 | The project is funded by the Environmental Agency of Norway. |
| 研究區域描述 | The survey is centred around the Oslofjord in southeast Norway. |
| 研究設計描述 | Sites are selected by two different methods, both aiming to target areas with high probability for occurrence of alien species, in particular new alien species. All sites based on the SSB grid for Norway, and formed as 250 x 250 meter squares. The automatically selected sites are drawn by an algorithm based on the following premises; (1) minimum eight single houses in the site (2) population density of minimum 30 and maximum 125 in the site (3) minimum 100 meters to the closest forested area (based on AR5 – the rationale for this was to ensure there was some nature in the site to which alien species could spread, as species in gardens or parks would not be included) (4) a weighted likelihood based on the modelled occurrence of alien plants from Olsen et al. (2017), i.e. higher likelihood to include sites in hot-spots for alien plants. The first two criteria resulted in many sites in residential areas with gardens, which was the intention, as imported garden plants are a very important source of alien species (Artsdatabanken 2023a). The manually selected sites are placed near potential pathways of introduction and spread of alien species that are not covered by the automatically selected sites. These sites are typically close to waste disposal sites with open depot for garden waste, transport hubs such as docks or freight terminals, or timber processing sites that are still or have previously imported timber from abroad (as timber can house several alien species of insects). |
參與計畫的人員:
取樣方法
One malaise trap per site
| 研究範圍 | Southeast Norway Sampling conducted in May to September |
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方法步驟描述:
- Sampling arthropods with malaisetraps, in 96% ethanol, emptied once every four weeks Straining the ethanol from the samples and adding control organisms (10 meal worms, 3 crickets and 10 bruchinae beetles). Lysis of the samples with ATL buffer and proteinase-K (100mL ATL = 1mL proteinase-K), incubated 3,5 hours while shaking (120 RPM) at 56 degrees celsius. DNA exctracted with Blood and Tissue kit (Quiagen) from 200 µL of the buffer solution. Amplifying COI with the primers BF3-BR2 (Elbrecht et al. 2019) in PCR (22 cycles) with overhang adaptors sequences in first run and Illumina-indexes in second run. Quality control of PCR products on a Tape Station (Agilent 4200) and cleaning with MAG-BIND RXN PURE PLUS. Sequencing with a Illumina NovaSeq machine at Norwegian Sequencing Centre (NSC) in Oslo.