Description
The initial goal of the project is to detect new alien species in early establishment phase in Norwegian nature. Insects and other arthropods have been sampled from urban and suburban sites in southeast-Norway. The trap collects flying insects passively in a bottle with 96% ethanol and is emptied once every four weeks, from May to September. All arthropods in the samples are identified by DNA-metabarcoding. DNA is extracted from the samples using a soft lysis method, which extracts DNA from the specimens without destroying them. The COI-region is then amplified from the extracted DNA and sequenced. The sequences are then filtered and quality checked and identified through matching with an in-house database of reference sequences.
Data Records
The data in this sampling event resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 974 records.
1 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versions
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Researchers should cite this work as follows:
Jacobsen R, Åström J (2024). Early detection of alien arthropod species in Norway. Version 1.0. Norwegian Institute for Nature Research. Samplingevent dataset. https://ipt.nina.no/resource?r=early_detection_alien_arthropods&v=1.0
Rights
Researchers should respect the following rights statement:
The publisher and rights holder of this work is Norwegian Institute for Nature Research. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
This resource has been registered with GBIF, and assigned the following GBIF UUID: 1baa2c29-e247-4158-8cd4-05b72db1dd4e. Norwegian Institute for Nature Research publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Norway.
Keywords
Samplingevent
Contacts
- Metadata Provider ●
- Originator ●
- Point Of Contact
https://orcid.org/0000-0003-4055-1096
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- Originator ●
- Point Of Contact
Geographic Coverage
As of 2023, the geographic scope is limited to the wider Oslo bay area.
| Bounding Coordinates | South West [58.993, 9.637], North East [60.273, 11.374] |
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Taxonomic Coverage
The sampling and bioinformatics focus on insects, but other classes of Arthropods also occur in the dataset.
| Kingdom | Metazoa |
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| Phylum | Arthropoda |
Temporal Coverage
| Start Date / End Date | 2021-05-05 / 2023-08-24 |
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Project Data
The main goal of the project is to detec new alien species in early establishment phase in Norwegian nature.
| Title | Early detection of alien arthropod species in Norway |
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| Funding | The project is funded by the Environmental Agency of Norway. |
| Study Area Description | The survey is centred around the Oslofjord in southeast Norway. |
| Design Description | Sites are selected by two different methods, both aiming to target areas with high probability for occurrence of alien species, in particular new alien species. All sites based on the SSB grid for Norway, and formed as 250 x 250 meter squares. The automatically selected sites are drawn by an algorithm based on the following premises; (1) minimum eight single houses in the site (2) population density of minimum 30 and maximum 125 in the site (3) minimum 100 meters to the closest forested area (based on AR5 – the rationale for this was to ensure there was some nature in the site to which alien species could spread, as species in gardens or parks would not be included) (4) a weighted likelihood based on the modelled occurrence of alien plants from Olsen et al. (2017), i.e. higher likelihood to include sites in hot-spots for alien plants. The first two criteria resulted in many sites in residential areas with gardens, which was the intention, as imported garden plants are a very important source of alien species (Artsdatabanken 2023a). The manually selected sites are placed near potential pathways of introduction and spread of alien species that are not covered by the automatically selected sites. These sites are typically close to waste disposal sites with open depot for garden waste, transport hubs such as docks or freight terminals, or timber processing sites that are still or have previously imported timber from abroad (as timber can house several alien species of insects). |
The personnel involved in the project:
Sampling Methods
One malaise trap per site
| Study Extent | Southeast Norway Sampling conducted in May to September |
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Method step description:
- Sampling arthropods with malaisetraps, in 96% ethanol, emptied once every four weeks Straining the ethanol from the samples and adding control organisms (10 meal worms, 3 crickets and 10 bruchinae beetles). Lysis of the samples with ATL buffer and proteinase-K (100mL ATL = 1mL proteinase-K), incubated 3,5 hours while shaking (120 RPM) at 56 degrees celsius. DNA exctracted with Blood and Tissue kit (Quiagen) from 200 µL of the buffer solution. Amplifying COI with the primers BF3-BR2 (Elbrecht et al. 2019) in PCR (22 cycles) with overhang adaptors sequences in first run and Illumina-indexes in second run. Quality control of PCR products on a Tape Station (Agilent 4200) and cleaning with MAG-BIND RXN PURE PLUS. Sequencing with a Illumina NovaSeq machine at Norwegian Sequencing Centre (NSC) in Oslo.
Additional Metadata
| Alternative Identifiers | https://ipt.nina.no/resource?r=early_detection_alien_arthropods |
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