Benthic biodiversity in four pairs of Norwegian lakes

Ocorrência
Versão mais recente published by Norwegian Institute for Nature Research on dez. 8, 2022 Norwegian Institute for Nature Research
Publication date:
8 de dezembro de 2022
Licença:
CC-BY 4.0

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Descrição

The aim of the project was to assess the effect of water-level regulation on diversity of lake littoral meiofauna and protists. Littoral fine-gravel bottoms of four pairs of Norwegian lakes were sampled in June and September 2021. Lakes were sampled in pairs that had similar water-quality but one of which was regulated and the other was unregulated. The bottom material was sampled at 30-40 cm depth, 1-5 m from the shoreline. Six replicate samples from each sampling point was taken by lowering a 25-cm diameter cylinder (12 cm high) to the bottom and 5 spoons of the bottom material from inside the cylinder was transferred to each sample bottle. No macroinvertebrates (size > 1 mm) were observed in the spoons before emptying them to the sample bottles. Water was filtrated out of the bottles using a 10 µm mesh, and then, samples were stored in 96-% ethanol. In total, the dataset contains 101 samples, including 5 control samples and 96 samples from the lakes. Taxonomic composition of bottom-dwelling meiofauna and protists was determined using DNA metabarcoding of the V4 fragment of the 18S rRNA gene. Taxa were identified using the PR2 reference database (protists) and NCBI GenBank nucleotide database (meiofauna). Caution should be exercised when interpreting occurrences of single species, as the DNA metabarcoding and bioinformatics may contain errors. The effect of regulation on species richness and change in community composition (Alpha-diversity) was assessed using mixed effects models.

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Occurrence (core)
39347
dnaDerivedData 
39347

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O editor e o detentor dos direitos deste trabalho é Norwegian Institute for Nature Research. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 536649eb-a529-4f6d-b97c-0c31b5f75a2d.  Norwegian Institute for Nature Research publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Norway.

Palavras-chave

Occurrence

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Contatos

Markus Majaneva
  • Originador
  • Ponto De Contato
Researcher
NINA
Trondheim
NO
Matthew Grainger
  • Provedor Dos Metadados
Researcher
NINA
Trondheim
NO
Roald Vang
  • Ponto De Contato
Section manager
NINA
Trondheim
NO

Cobertura Geográfica

Meråker, Orkland, Melhus, Leksvik, Inderåy, Fosen and Skaun Municipalities in Trøndelag County, Norway

Coordenadas delimitadoras Sul Oeste [62,714, 6,24], Norte Leste [66,249, 15,029]

Cobertura Taxonômica

Benthic Invertebrates

Filo Apicomplexa, Apusomonadidae, Centroheliozoa, Cercozoa, Chlorophyta, Choanoflagellida, Chrompodellids, Ciliophora, Conosa, Cryptophyta, Dinoflagellata, Discoba, Fungi, Glaucophyta, Haptophyta, Hemimastigophora, Hilomonadea, Katablepharidophyta, Lobosa, Mesomycetozoa, Metamonada, Metazoa, Ochrophyta, Opalozoa, Perkinsea, Pseudofungi, Rhodophyta, Sagenista, Stramenopiles, Streptophyta, Telonemia

Cobertura Temporal

Data Inicial / Data final 2021-06-08 / 2021-09-15

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Benthic biodiversity in four Norwegian reservoirs

O pessoal envolvido no projeto:

Markus Majaneva

Métodos de Amostragem

Lakes were sampled in pairs that had similar water-quality but one of which was regulated and the other was unregulated. The bottom material was sampled at 30-40 cm depth, 1-5 m from the shoreline. Six replicate samples from each sampling point was taken by lowering a 25-cm diameter cylinder (12 cm high) to the bottom and 5 spoons of the bottom material from inside the cylinder was transferred to each sample bottle. No macroinvertebrates (size > 1 mm) were observed in the spoons before emptying them to the sample bottles. Water was filtrated out of the bottles using a 10 µm mesh, and then, samples were stored in 96-% ethanol.

Área de Estudo Trøndelag County Norway, in the Meråker, Orkland, Melhus, Leksvik, Inderøy, Fosen and Skaun Municipalities

Descrição dos passos do método:

  1. Taxonomic composition of bottom-dwelling meiofauna and protists was determined using DNA metabarcoding of the V4 fragment of the 18S rRNA gene. Taxa were identified using the PR2 reference database (protists) and NCBI GenBank nucleotide database (meiofauna).

Citações bibliográficas

Metadados Adicionais