Benthic biodiversity in four pairs of Norwegian lakes

出現紀錄
最新版本 published by Norwegian Institute for Nature Research on 12月 8, 2022 Norwegian Institute for Nature Research
發布日期:
2022年12月8日
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CC-BY 4.0

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說明

The aim of the project was to assess the effect of water-level regulation on diversity of lake littoral meiofauna and protists. Littoral fine-gravel bottoms of four pairs of Norwegian lakes were sampled in June and September 2021. Lakes were sampled in pairs that had similar water-quality but one of which was regulated and the other was unregulated. The bottom material was sampled at 30-40 cm depth, 1-5 m from the shoreline. Six replicate samples from each sampling point was taken by lowering a 25-cm diameter cylinder (12 cm high) to the bottom and 5 spoons of the bottom material from inside the cylinder was transferred to each sample bottle. No macroinvertebrates (size > 1 mm) were observed in the spoons before emptying them to the sample bottles. Water was filtrated out of the bottles using a 10 µm mesh, and then, samples were stored in 96-% ethanol. In total, the dataset contains 101 samples, including 5 control samples and 96 samples from the lakes. Taxonomic composition of bottom-dwelling meiofauna and protists was determined using DNA metabarcoding of the V4 fragment of the 18S rRNA gene. Taxa were identified using the PR2 reference database (protists) and NCBI GenBank nucleotide database (meiofauna). Caution should be exercised when interpreting occurrences of single species, as the DNA metabarcoding and bioinformatics may contain errors. The effect of regulation on species richness and change in community composition (Alpha-diversity) was assessed using mixed effects models.

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亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。

Occurrence (核心)
39347
dnaDerivedData 
39347

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此資料的發布者及權利單位為 Norwegian Institute for Nature Research。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

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關鍵字

Occurrence

外部資料

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聯絡資訊

Markus Majaneva
  • 出處
  • 連絡人
Researcher
NINA
Trondheim
NO
Matthew Grainger
  • 元數據提供者
Researcher
NINA
Trondheim
NO
Roald Vang
  • 連絡人
Section manager
NINA
Trondheim
NO

地理涵蓋範圍

Meråker, Orkland, Melhus, Leksvik, Inderåy, Fosen and Skaun Municipalities in Trøndelag County, Norway

界定座標範圍 緯度南界 經度西界 [62.714, 6.24], 緯度北界 經度東界 [66.249, 15.029]

分類群涵蓋範圍

Benthic Invertebrates

Phylum Apicomplexa, Apusomonadidae, Centroheliozoa, Cercozoa, Chlorophyta, Choanoflagellida, Chrompodellids, Ciliophora, Conosa, Cryptophyta, Dinoflagellata, Discoba, Fungi, Glaucophyta, Haptophyta, Hemimastigophora, Hilomonadea, Katablepharidophyta, Lobosa, Mesomycetozoa, Metamonada, Metazoa, Ochrophyta, Opalozoa, Perkinsea, Pseudofungi, Rhodophyta, Sagenista, Stramenopiles, Streptophyta, Telonemia

時間涵蓋範圍

起始日期 / 結束日期 2021-06-08 / 2021-09-15

計畫資料

無相關描述

計畫名稱 Benthic biodiversity in four Norwegian reservoirs

參與計畫的人員:

Markus Majaneva

取樣方法

Lakes were sampled in pairs that had similar water-quality but one of which was regulated and the other was unregulated. The bottom material was sampled at 30-40 cm depth, 1-5 m from the shoreline. Six replicate samples from each sampling point was taken by lowering a 25-cm diameter cylinder (12 cm high) to the bottom and 5 spoons of the bottom material from inside the cylinder was transferred to each sample bottle. No macroinvertebrates (size > 1 mm) were observed in the spoons before emptying them to the sample bottles. Water was filtrated out of the bottles using a 10 µm mesh, and then, samples were stored in 96-% ethanol.

研究範圍 Trøndelag County Norway, in the Meråker, Orkland, Melhus, Leksvik, Inderøy, Fosen and Skaun Municipalities

方法步驟描述:

  1. Taxonomic composition of bottom-dwelling meiofauna and protists was determined using DNA metabarcoding of the V4 fragment of the 18S rRNA gene. Taxa were identified using the PR2 reference database (protists) and NCBI GenBank nucleotide database (meiofauna).

引用文獻